Variable blood processing procedures contribute to plasma proteomic variability

Table 2 Top ten proteins with the most statistically significant associations (the lowest BH-FDR adjusted p-values) for the interaction between temperature and hold time in multi-donor blood processing study

Accession	Description	logFC	AveExpr	BH-FDR
P02788	LTF: lactotransferrin	− 1.03	7.29	0.0019
O14511-2	NRG2: Isoform 2 Of Pro-Neuregulin-2, membrane-bound isoform	− 0.78	7.36	0.0022
P23528	CFL1: cofilin-1	− 0.67	6.59	0.0033
P52209-2	PGD: isoform 2 of 6-phosphogluconate dehydrogenase, decarboxylating	− 0.97	6.31	0.0090
P06744	GPI: glucose-6-phosphate isomerase	− 0.93	6.29	0.0090
P30740	SERPINB1: leukocyte elastase inhibitor	− 0.93	6.16	0.0115
P04083	ANXA1: annexin A1	− 0.82	6.31	0.0115
P00558-2	PGK1: isoform 2 of phosphoglycerate kinase 1	− 0.95	6.66	0.0187
P02655	APOC2: apolipoprotein C-II	− 0.23	9.87	0.0211
P02730	SLC4A1: band 3 anion transport protein	− 1.02	6.15	0.0211

Column “logFC” represents estimated fold change on log base 2 scale due to one day delay in processing at 4C, as compared to room temperature (shown in Table 1). Negative sign for “logFC” indicates lower rate of change with time at lower temperature. Column “AveExpr” represents average expression level of each protein on the scale of log base 2 CPM (as further explained in Additional file 1: Section S1)

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