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Fig. 2 | Clinical Proteomics

Fig. 2

From: Robust optimization of SWATH-MS workflow for human blood serum proteome analysis using a quality by design approach

Fig. 2

Contour plots showing the mean number of proteins (in red) as a function of the Number of SWATH Windows and the MS/MS accumulation time. The blue regions indicate areas of high variability (coefficient of variation > 30%). The pink regions indicate areas of low yield (less than 300 proteins recovered). The remaining space (in white) marks a safe operating region where we are likely to meet both constraints recovering higher numbers of proteins with reduced variability. The goal of robust optimization is to identify such regions

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