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Fig. 3 | Clinical Proteomics

Fig. 3

From: Robust optimization of SWATH-MS workflow for human blood serum proteome analysis using a quality by design approach

Fig. 3

Mean number of proteins recovered as a function of MS/MS accumulation time and number of SWATH windows. More than 300 proteins were recovered at settings of both: 80 SWATH windows and an Accumulation time of 60 ms; 100 SWATH windows and an Accumulation time of 40 ms. While the number of proteins was maximized at 80 SWATH windows and an Accumulation time of 60 ms, the choice of 100 SWATH windows and 40 ms for the accumulation time was more robust—giving consistently over 300 proteins—with a cycle time of 4 s permitting satisfactory peak integration

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Clinical Proteomics

ISSN: 1559-0275