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Table 1 Differentially expressed protein spots in the FT compared to the TP, identified by LC–MS/MS

From: Proteome profiling of human placenta reveals developmental stage-dependent alterations in protein signature

Spota

Protein name

Mrb

pIb

Unusedc

%Cov (95)d

Accession No

Peptides (95%)e

1

78 kDa glucose-regulated protein

OS = Homo sapiens GN = HSPA5 PE = 1 SV = 2

72.4/ 78

5.07/ 5.2

74.43

69.1

sp|P11021|GRP78

HUMAN

131

2

Serotransferrin

OS = Homo sapiens GN = TF PE = 1 SV = 3

79.3/ 83

6.08/ 6.7

87.78

61.8

sp|P02787|TRFE

HUMAN

56

3

Serum albumin

OS = Homo sapiens GN = ALB PE = 1 SV = 2

67/ 75

5.7/ 6.1

88.09

73.4

sp|P02768|ALBU

HUMAN

112

4

Protein disulfide-isomerase A3

OS = Homo sapiens GN = PDIA3 PE = 1 SV = 4

58/ 60

5.8/ 6.2

91.24

79.6

sp|P30101|PDIA3

HUMAN

231

5

Keratin, type II cytoskeletal 1

OS = Homo sapiens GN = KRT8 PE = 1 SV = 7

51.3/ 50

5.5/ 5.9

47.16

69.2

sp|P05787|K2C8

HUMAN

79

6

Alpha-enolase

OS = Homo sapiens GN = ENO1 PE = 1 SV = 2

47.3/ 50

6.98/ 7.3

50

65.9

sp|P06733|ENOA

HUMAN

57

7

Actin, cytoplasmic 2

OS = Homo sapiens GN = ACTG1 PE = 1 SV = 1

42.1/ 40

5.3/ 5.7

30.21

52.8

sp|P63261|ACTG

_HUMAN

21

8

Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial

OS = Homo sapiens GN = ECH1 PE = 1 SV = 2

34.4/ 30

6.61/ 6.5

48.18

71.3

sp|Q13011|ECH1

HUMAN

92

9

Peroxiredoxin-4

OS = Homo sapiens GN = PRDX4 PE = 1 SV = 1

24.9/ 27

5.73/ 6.3

32.51

67.2

sp|Q13162|PRDX4

_HUMAN

102

10

Endoplasmic reticulum resident protein 29

OS = Homo sapiens GN = ERP29 PE = 1 SV = 4

28.5/ 25

6.7/ 6.5

31.12

61.3

sp|P30040|ERP29

HUMAN

35

11

Enoyl-CoA hydratase, mitochondrial

OS = Homo sapiens GN = ECHS1 PE = 1 SV = 4

26.1/ 25

5.9/ 6.4

35.79

77.6

sp|P30084|ECHM

HUMAN

65

12

Chorionic Somatomammotropin Hormone 2

OS = Homo sapiens GN = CSH2 PE = 1 SV = 1

18.8/ 25

5.34/ 5.6

23.98

62.7

sp|P0DML3|CSH2

HUMAN

56

13

Peroxiredoxin-2

OS = Homo sapiens GN = PRDX2 PE = 1 SV = 5

22.04/ 20

5.65/ 5.9

45.98

75.8

sp|P32119|PRDX2

HUMAN

69

14

Myeloid-derived growth factor

OS = Homo sapiens GN = MYDGF PE = 1 SV = 1

18.7/ 17

6.31/ 7.1

22.63

56.7

sp|Q969H8|MYDGF

HUMAN

32

15

Fatty acid-binding protein, epidermal

OS = Homo sapiens GN = FABP5 PE = 1 SV = 3

15.16/ 16

6.6/ 6.9

15.88

60.7

sp|Q01469|FABP5

HUMAN

19

16

Hemoglobin subunit gamma-1

OS = Homo sapiens GN = HBG1 PE = 1 SV = 2

16.1/ 15

6.7/ 7.5

39.62

95.2

sp|P69891|HBG1

HUMAN

120

17

Fatty acid-binding protein,adipocyte

OS = Homo sapiens GN = FABP4 PE = 1 SV = 3

14.71/ 16

6.59/ 6.9

26.15

66.7

sp|P15090|FABP4

HUMAN

35

18

Keratin, type II cytoskeletal 8

OS = Homo sapiens GN = KRT8 PE = 1 SV = 7

51.3/ 50

5.5/ 6

71.34

60.5

sp|P05787|K2C8

HUMAN

117

19

LDLR chaperone MESD

OS = Homo sapiens GN = MESDC2 PE = 1 SV = 2

26.07/ 19

6.1/ 6.6

24.35

57.3

sp|Q14696|MESD

HUMAN

39

20

Keratin, type I cytoskeletal 9

OS = Homo sapiens GN = KRT9 PE = 1 SV = 3

62.06/ 51

5.04/ 5.8

20.21

21.7

sp|P35527|K1C9

HUMAN

11

  1. LC–MS/MS: Liquid chromatography-tandem mass spectrometry; Mr: the average mass of the protein; pI: isoelectronic point; FT: First trimester placenta; TP: Term placenta
  2. aSpot numbers are the same as the spot labels in Fig. 2
  3. bTheoretical/ Experimental Mr (KD); bTheoretical/ Experimental pI
  4. CUnused is the score computed by the software according to the number of good peptides (the higher the score, the higher the confidence that this protein was identified)
  5. d %Cov is the Coverage percentage
  6. ePeptides(95%) is the number of the identified peptides with a confidence higher than 95%