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Fig. 5 | Clinical Proteomics

Fig. 5

From: Mass spectrometry-based proteomics in basic and translational research of SARS-CoV-2 coronavirus and its emerging mutants

Fig. 5

Development of IP-SRM assays. A Selection of proteotypic peptides of SARS-COV-2 nucleoprotein based on experimental shotgun mass spectrometry data. Peptide identification by shotgun mass spectrometry provided tryptic peptides with the highest MS1 intensities (our unpublished data), and three peptides AYNVTQAFGR, DQVILLNK and GFYAEGSR were selected. MaxQuant search revealed the most intense y-ion transitions of peptide AYNVTQAFGR. Finally, optimization of the nanoflow liquid chromatography and targeted mass spectrometry parameters generated a quantitative SRM assay. B IP-SRM included an antibody-mediated enrichment of NCAP_SARS2 followed by proteomic sample preparation and SRM quantification

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Clinical Proteomics

ISSN: 1559-0275