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Fig. 2 | Clinical Proteomics

Fig. 2

From: A validated analysis pipeline for mass spectrometry-based vitreous proteomics: new insights into proliferative diabetic retinopathy

Fig. 2

Experiment 2 design. Four 10-plexes (2.1–2.4), each containing a combination of control, PDR, and pool samples, were subjected to TMT-MS on the same day. Pool 1 aliquots were made from the same pool of control samples used in experiment 1 and served as technical replicates. Pool 2 aliquots were derived from a mixture of control and PDR samples in order to reflect the complexity of individual samples used in experiment 2. Data from plexes 2.1–2.4 were then aggregated into a normalized expression matrix. Matrix data were used for statistical and bioinformatic analyses

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