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Fig. 2 | Clinical Proteomics

Fig. 2

From: A SISCAPA-based approach for detection of SARS-CoV-2 viral antigens from clinical samples

Fig. 2

Experimental workflow for enrichment of nucleocapsid peptides using SISCAPA. Anti-peptide antibodies generated against three SARS-CoV-2 viral nucleocapsid protein-derived peptides (NPANNAAIVLQLPQGTTLPK, DGIIWVATEGALNTPK and ITFGGPSDSTGSNQNGER) were used to enrich from SARS-CoV-2 positive or negative nasopharyngeal swab samples. The anti-peptide antibodies were crosslinked to MSIA tips and peptides were enriched on MSIA tips using the Versette liquid handler platform as indicated. The enriched peptides were analyzed individually by targeted PRM analysis on an Orbitrap Eclipse mass spectrometer

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Clinical Proteomics

ISSN: 1559-0275