Fig. 3
From: A SISCAPA-based approach for detection of SARS-CoV-2 viral antigens from clinical samples
PRM analysis of viral nucleocapsid peptides after enrichment. A A representative figure of Skyline traces for NPANNAAIVLQLPQGTTLPK, DGIIWVATEGALNTPK and ITFGGPSDSTGSNQNGER peptides and their retention times. To determine the limit of detection (LOD), NPANNAAIVLQLPQGTTLPK, DGIIWVATEGALNTPK and ITFGGPSDSTGSNQNGER peptides were spiked into PBS and enrichment was done using the SISCAPA workflow. B Regression analysis presented in the figure demonstrates linearity of peak areas with the amount of spiked-in peptides as indicated. The transition ratios for selected fragment ions was reproducible regardless of amount of the analyte. Peak areas across all the peptide amounts spiked are shown in Additional file 1: Fig.S1. C The CVs calculated for three independent experiments for each peptide (each peptide analyzed in triplicate) are shown