Table 2 Variability (reported as CV) for SISCAPA assay performed on 3 separate sets of pooled RT-PCR positive nasopharyngeal swab samples (Ct value < 24). The total area was considered for calculating the mean and standard deviation

Set 1

 Mean

1.64E + 08

4.90E + 07

6.04E + 06

 SD

2.63E + 07

3.37E + 06

6.28E + 05

 CV

16.04

6.88

10.38

 Mean

1.62E + 08

5.15E + 07

5.69E + 06

Set 2

 SD

2.13E + 07

4.30E + 06

5.50E + 05

 CV

13.14

8.34

9.66

 Mean

1.87E + 08

4.28E + 07

6.27E + 06

Set 3

 SD

1.06E + 07

3.60E + 06

3.90E + 05

 CV

5.64

8.41

6.21