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Fig. 5 | Clinical Proteomics

Fig. 5

From: Combined proteomic/transcriptomic signature of recurrence post-liver transplantation for hepatocellular carcinoma beyond Milan

Fig. 5

Verification of ALDH1A1 and LGALS3 protein expression changes in HCC tumour explants. A Shows the immunoblot bands of ALDH1A1, LGALS3, and GAPDH in recurrent (n = 7) and non-recurrent (n = 4) HCC patients. Cropped images of the blots were used in order to improve the clarity and conciseness of the presentation. The protein expression of ALDH1A1 (B) and LGALS3 (C) were measured by densitometry and normalized to GAPDH. The Pearson correlations between Western Blot and mass spectrometry log2-transformed LFQ protein intensity values of ALDH1A1 and LGALS3 were evaluated (D). The Western blot LGALS3/ALDH1A1 ratio was also calculated (E). *P < 0.05. Data are reported as mean ± standard error. LFQ, label-free quantification; LGALS3, galectin-3; ALDH1A1, retinal dehydrogenase 1. LGALS3, galectin-3; LGALS3BP, galectin-3-binding protein; ALDH1A1, retinal dehydrogenase 1

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Clinical Proteomics

ISSN: 1559-0275