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Fig. 1 | Clinical Proteomics

Fig. 1

From: 13C15N: glucagon-based novel isotope dilution mass spectrometry method for measurement of glucagon metabolism in humans

Fig. 1

A schematic workflow for targeted LC–MS/MS analysis of glucagon tracers from clinical samples. Subjects were infused with stable isotopically labeled heavy tracers of glucagon followed by blood collection at specified time intervals. The plasma samples were then subjected to anti-glucagon antibody-based enrichment of endogenous and heavy glucagon tracers. PRM-based targeted LC–MS/MS analysis was carried using high resolution Q-Exactive Orbitrap mass spectrometer for measurement of glucagon kinetics

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Clinical Proteomics

ISSN: 1559-0275