Skip to main content
Fig. 3 | Clinical Proteomics

Fig. 3

From: 13C15N: glucagon-based novel isotope dilution mass spectrometry method for measurement of glucagon metabolism in humans

Fig. 3

Calibration curves for quantitation of glucagon tracers in human plasma. The area ratio of glucagon tracers (FF and FFLA glucagon) to IS (F22 glucagon) was determined from the extracted ion chromatograms (EIC) at MS1 and MS2 levels. For calibration curve of FF glucagon, the combined EIC of m/z 876.17; 876.42; 876.68 at MS1 (A) and m/z 1008.82; 1009.16; 1046.85; 1047.18; 1090.20; 1090.8 at MS2 (B) was used while For FFLA glucagon, the combined EIC of m/z 878.68; 878.93; 879.18 at MS1 (C) and m/z 1012.17; 1012.84; 1049.86; 1050.19; 1093.88; 1094.21 at MS2 (D). The area ratios were plotted against concentration (range: 1.56–100 pg/ml)

Back to article page

Clinical Proteomics

ISSN: 1559-0275