Fig. 3
From: Systematic evaluation and optimization of protein extraction parameters in diagnostic FFPE specimens
Buffer composition and detergents. Subplots report the different concentrations and quantities. A n = 6, medians with interquartile range of the respective data as whiskers; Com = commercial buffer EXB; Z = buffer containing Zwittergent 3-16; Z + S = buffer containing Zwittergent and SDS; S = buffer containing SDS only; S-T = buffer containing SDS and lower concentration of Tris; RG and RG-T = in analogy for RapiGest; please refer to the text for the detailed buffer compositions; B Effects of SDS concentration variation (w/v); n = 3, n = 4 for some proteins, means ± standard deviation; C High-performance liquid chromatography elution profile (total ion current) of sample preparation with buffer and precipitation variation for LC–MS