Fig. 5
From: Systematic evaluation and optimization of protein extraction parameters in diagnostic FFPE specimens
LC–MS comparison of the optimized versus the standard qProteome protocol. A Extracts from two biological replicates (different tissue blocks, different tumors) were analyzed (top and bottom row). (Left): Elution profiles (total ion current); (Right): Venn diagrams showing the distribution of the identified proteins with membrane proteins as separate subset; B Western blot comparison between the commercial qProteome protocol and buffer (standard) with the optimized protocol; n = 6, medians with interquartile ranges of the respective data as whiskers