Table 2 Mass-spectrometric comparison of the two key buffers and precipitation modes
From: Systematic evaluation and optimization of protein extraction parameters in diagnostic FFPE specimens
Buffer | Commercial (Com) | Custom (S*) | ||
|---|---|---|---|---|
Precipitation mode | Methanol | Acetone | Methanol | Acetone |
Peptides identified | 7595 | 6048 | 8634 | 8210 |
Proteins identified | 1258 | 1064 | 1347 | 1361 |
Membrane proteins | 164 | 124 | 184 | 167 |
 % of all proteins | 13.0 | 11.7 | 13.7 | 12.3 |
Cumul. intensity [arb.] | 8.96 E + 07 | 6.60 E + 07 | 1.10 E + 08 | 1.01 E + 08 |
 Median | 1.34 E + 04 | 1.19 E + 04 | 1.44 E + 04 | 1.32 E + 04 |
 Mean | 7.12 E + 04 | 6.20 E + 04 | 8.19 E + 04 | 7.45 E + 04 |