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Fig. 1 | Clinical Proteomics

Fig. 1

From: Transcriptome profiling and proteomic validation reveals targets of the androgen receptor signaling in the BT-474 breast cancer cell line

Fig. 1

Project pipeline. A Workflow of the transcriptomic analysis of hormonally treated BT474 cells. Cells were cultured and treated with hormones or ethanol (used as control) for 24 h. Cell pellets were collected and subjected to total RNA isolation. GSEA and Cytoscape software was used for the functional and pathway analysis of the differentially expressed genes. DHT dihydrotestosterone (10 nM), PROG progesterone (10 nM). B Principal Component Analysis (PCA) of BT474 RNA sequencing in three treatment conditions (Ethanol, DHT, and PROG). Each condition was performed in triplicates and groups clearly cluster. DHT dihydrotestosterone (10 nM), PROG progesterone (10 nM). C Experimental workflow of targeted PRM proteomics and data analysis for the validation of select androgen-regulated proteins in the BT474 cell line. For details see under “methods”

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