Fig. 2

Hormonally regulated changes in the BT474 transcriptome. A Pie charts illustrating the different transcript types that are hormonally regulated in BT474 cells. In both conditions, DHT- and PROG-treated cells, 79% of the transcripts are protein-coding, 5% long non-coding RNAs (lncRNA), 2% microRNAs (miRNA), 2% small non-coding RNA (ncRNA), 2% pseudogenes, and the remaining 11% belong to other categories and gene ID duplications. *snoRNA, snRNA, scaRNA, scRNA, miscRNA. **Polymorphic_pseudogene, processed_pseudogene, transcribed_processed_pseudogene, transcribed_unitary_pseudogene, transcribed_unprocessed_ pseudogene, unitary_pseudogene, unprocessed_pseudogene. ***TEC (To be experimentally confirmed), ribosome The transcript categorization was performed on Ensembl website, using the bioinformatics tool, Biomart. B Histograms demonstrating the FC (fold change) distribution of DEGs in the transcript list, C Volcano plots illustrating the most significant differentially expressed genes. Red color shows the upregulated genes and blue color shows the downregulated genes. D Heatmap illustrating the differential expression profiles in DHT-treated and PROG-treated cells, respectively, compared to controls. Red color represents the upregulated genes, whereas the blue color represents the downregulated genes. Grey color corresponds to the genes that were not expressed, or the ratio could not be assessed in this experiment. All graphs were constructed in Perseus software using the log₂FC values derived from the expression comparison between the hormone-treated and control cells. E, F Venn diagram representing the number and overlapping of DEGs in DHT-treated BT474 cells with known AR-regulated and cancer/BCa-related genes. The identification of the cancer/BCa related enriched genes were identified with the Gene Ontology annotation (E) and the KEGG analysis (F) of DAVID (2021 Update) online tool. G Comparison of gene set numbers identified in GSEA analysis of the RNA-seq experiment in DHT and PROG-treated BT474 cells. For the GSEA (v.3.0) analysis, only gene sets that contained 15–200 genes were used (q-value < 0.1). DHT dihydrotestosterone (10 nM), PROG progesterone (10 nM) *NES: normalized enrichment score