Fig. 2
From: Many kinds of oxidized proteins are present more in the urine of the elderly
Oxidations and AA substitutions changing with aging in discovery cohort. A Unsupervised cluster analysis results of the total modifications for three groups samples. Children group covers with green, and young age group covers with yellow, the senior group covers with blue. B Heatmap depicting the levels of differentially identified modifications in discover cohorts with different age groups. The graphs show the relative intensity of differentially expressed modifications. Proteins included in the heatmap meet the requirement that fold change >2 or <0.5 and p-value (t test) of <0.05 comparing different age samples. Color bar represents the relative intensity of identified proteins from −4 to 4. C and D Heatmap depicting the levels of differentially identified oxidations and AA substitutions among different ages. The graphs show the relative intensity of differentially expressed modifications. Color bar represents the relative intensity of identified proteins from −6 to 6. E and F The scatter plot graphs showing all differentially expressed oxidations that are potential diagnostic markers for aging. Comparison of oxidation differences among children group, young age, and senior group. The horizontal line in the middle of the data represents the median, the upper and lower color lines represent the data quartile range, and the upper part of the data represents the significance between groups. Data are presented as mean ± SD. As the number of labels increases, the significance increases. (*p <0.05, **p <0.01, ***p <0.005, ****p <0.001). G 14 modifications changed trend with age. The expression of 11 oxidation modifications and 3 AA substitutions increased with aging. H and I The scatter plot histogram graphs showing 11 differentially expressed oxidation modifications and 3 AA substitutions that are potential diagnostic markers for aging. Comparison of oxidation differences among children group, young age, and senior group. The horizontal line in the middle of the data represents the median, the upper and lower color lines represent the data quartile range, and the upper part of the data represents the significance between groups. Data are presented as mean ± SD. As the number of labels increases, the significance increases. (*p <0.05, **p <0.01, ***p <0.005, ****p <0.001). J The ROC plot of 4 oxidations and an AA substitution