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Fig. 2 | Clinical Proteomics

Fig. 2

From: Combination of automated sample preparation and micro-flow LC–MS for high-throughput plasma proteomics

Fig. 2

Optimization of the automated plasma sample preparation workflow. A Comparison of reduction and alkylation conditions for the number of identified protein groups and peptides. B Comparison of enzymes used in combination or alone. C Identification reproducibility of three batches of the same plasma sample processed at the same plate. D CVs of all quantified proteins were calculated for the 3 replicates. Proteins with CV < 20% are colored in blue and those with CV > 20% in gray. E Distribution of PSMs in each missed cleavages% levels. F Correlation of the protein intensities between replicate 1 and replicate 3. Correlation of protein intensities between the other replicates were appended in Figure S2

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