Fig. 3

Performance of the automated plasma proteome profiling procedure. A Comparison of identified proteins number of AMTK with “Optimized reagents” and “EasyPep reagent kit” (n = 4). B Four randomly selected samples from one plate were subjected to micro-flow LC–MS analysis. C Average number of protein groups and unique peptides identified of the same sample processed on three different days (day 1, day 17, and day 22) over a period of a month under optimized conditions and subjected to micro-flow LC–MS analysis. Error bars represent standard deviations from three technical replicates. D Correlations of the protein intensity between each of the two samples during the three days. E CVs of all quantified proteins were calculated for the 4 replicates. Proteins with CV < 20% are colored in blue and those with CV > 20% in gray. F The dynamic range of plasma proteome, FDA-approved biomarkers highlighted in red. G The CVs of 35 FDA-approved biomarkers from 4 replicates