Fig. 1

Agarose gel electrophoresis and GelRed Staining of PCR products from mitochondrial DNA in human plasma with mitochondrial MT-ND1 specific primers. A PCR analysis of representative COVID-19, ICU-ARDS and NHP plasma samples; B the variation in COVID-19 and ICU-ARDS PCR amplification. The PCR conditions were 40 cycles, lid temp 105 °C, 25 µL reaction volume, 94 °C melting (30 s), 58 °C annealing (1 min), 72 °C extension (1 min). Template DNA was extracted from plasma with Trizol. Serum from the Luxembourg cohort was used as Normal Human Plasma (NHP) healthy controls