- Original Article
- Open Access
Assessment of protein stability in cerebrospinal fluid using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry protein profiling
Clinical Proteomics volume 2, pages91–101(2006)
Recent studies have evaluated proper acquisition and storage procedures for the use of serum or plasma for mass spectrometry (MS)-based proteomics. The present study examines the proteome stability of human cerebrospinal fluid (CSF) over time at 23°C (room temperature) and 4°C using surface-enhanced laser desorption/ionization time-of-flight MS. Data analysis revealed that statistically significant differences in protein profiles are apparent within 4 h at 23°C and between 6 and 8 h at 4°C. Inclusion of protease and phosphatase inhibitor cocktails into the CSF samples failed to significantly reduce proteome alterations over time. We conclude that MS-based proteomic analysis of CSF requires careful assessment of sample collection procedures for rapid and optimal sample acquisition and storage.
Petricoln, E. F., Ardekani, A. M., Hitt, B. A., et al. (2002) Use of proteomic patterns in serum to identify ovarian cancer. Lancet 359, 572–577.
Rosty, C., Christa, L., Kuzdzal, S., et al. (2002) Identification of hepatocarcinoma-intestine-pancreas/ pancreatitis-associated protein I as a biomarker for pancreatic ductal adenocarcinoma by protein biochip technology. J. Cancer Res. 62, 1868–1875.
Rogers, M. A., Clarke, P., Noble, J., et al. (2003) Proteomic profiling of urinary proteins in renal cancer by surface enhanced laser desorption Ionization and neural-network analysis: Identification of key issues affecting potential clinical utility. Cancer Res. 63, 6971–6983.
Carrette, O., Demalte, I., Scherl, A., et al. (2003) A panel of cerebrospinal fluid potential biomarkers for the diagnosis of Alzheimer's disease. Proteomics 3, 1486–1494.
Xiao, Z., Luke, B. T., Izmirlian, G., et al. (2004) Serum proteomic profiles suggest celecoxib-modulated targets and response predictors. Cancer Res. 64, 2904–2909.
Kozak, K. R., Su, F., Whitelegge, J. P., Faull, K., Reddy, S., and Farias-Eisner, R. (2005) Characterization of serum biomarkers for detection of early stage ovarian cancer. Proteomics 5, 4589–4596.
Norwitz, E. R., Tsen, L. C., Park, J. S., et al. (2005) Discriminatory proteomic biomarker analysis identifies free hemoglobin in the cerebrospinal fluid of women with severe preeclampsia. Am. J. Obstet. Gynecol. 193, 957–964.
Woodrum, D., French, C., and Shamel, L. B. (1996) Stability of free prostate-specific antigen in serum samples under a variety of sample collection and sample storage conditions. Urology 48, 33–39.
Raabe, A., Kopetsch, O., Gross, U., Zimmermann, M., and Gebhart, P. (2003) Measurements of serum S-100B protein: effects of storage time and temperature on pre-analytical stability. Clin. Chem. Lab. Med. 41, 700–703.
Delatour, T. (2004) Performance of quantitative analyses by liquid chromatography-electrospray ionisation tandem mass spectrometry: from external calibration to isotopomer-based exact matching. Anal. Bioanal. Chem. 380, 515–523.
Spruessel, A., Steimann, G., Jung, M., et al. (2004) Tissue ischemia time affects gene and protein expression patterns within minutes following surgical tumor excision. Biotechniques 36, 1030–1037.
West-Nielsen, M., Hogdall, E. V., Marchiori, E., Hogdall, C. K., Schou, C., and Heegaard, N. H. H. (2005) Sample handling for mass spectrometric proteomic investigations of human sera. Anal. Chem. 77, 5114–5123.
Hesse, C., Larsson, H., Fredman, P., et al. (2000) Measurement of apolipoprotein E (apoE) in cerebrospinal fluid. Neurochem. Res. 25, 511–517.
Semmes, O. J., Feng, Z., Adam, B.-L., et al. (2005) Evaluation of serum protein profiling by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry for the detection of prostate cancer: I. Assessment of platform reproducibility. Clin. Chem. 51, 102–112.
Carrette, O., Burkhard, P. R., Hughes, S., Hochstrasser, D. F., and Sanchez, J. C. (2005) Truncated cystatin C in cerebrospinal fluid: technical artefact or biological process? Proteomics 5, 3060–3065.
Yuan, X., Russell, T., Wood, G., and Desiderio, D. M. (2002) Analysis of the human lumbar cerebrospinal fluid proteome. Electrophoresis 23, 1185–1196.
Davidsson, P., Sjogren, M., Andreasen, N., et al. (2002) Studies of the pathophysiological mechanisms of frontotemporal dementia by proteome analysis of CSF proteins. Mol. Brain Res. 109, 128–133.
Ramstrom, M., Ivonin, I., Johansson, A., et al. (2005) Cerebrospinal fluid protein patterns in neurodegenerative disease revealed by liquid chromatography-Fourier transform ion cyclotron resonance mass spectrometry. Proteomics 4, 4010–4018.
Levine, J., Panchalingam, K., McClure, R. J., Gershon, S., and Pettegrew, J. W. (2000) Stability of CSF metabolites measured by proton NMR. J. Neural Transm. 107, 843–848.
Zhang, J., Goodlett, D. R., Peskind, E. R., et al. (2005) Quantitative proteomic analysis of age-related changes in human cerebrospinal fluid. Neurobiol. Aging 26, 207–227.
Maccarrone, G., Birg, I., Malisch, E., et al. (2004) In-depth analysis of the human CSF proteome using protein prefractionation. Clin. Proteomics 1, 333–364.
Hulmes, J. D., Bethea, D., Ho, K., et al. (2004) An investigation of plasma collection, stabilization, and storage procedures for proteomic analysis of clinical samples. Clin. Proteomics 1(1), 17–31.
Marshall, J., Kupchak, P., Zhu, W., et al. (2003) Processing of serum proteins underlies the mass spectral fingerprinting of myocardial infarction. J. Proteome Res. 2, 361–372.
You, J.-S., Gelfanova, V., Knierman, M. D., Witzmann, F. A., Wang, M., and Hale, J. E. (2005) The impact of blood contamination on the proteome of cerebrospinal fluid. Proteomics 5, 290–296.
About this article
Cite this article
Ranganathan, S., Polshyna, A., Nicholl, G. et al. Assessment of protein stability in cerebrospinal fluid using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry protein profiling. Clin Proteom 2, 91–101 (2006). https://doi.org/10.1385/CP:2:1:91
- Phosphatase Inhibitor
- Relative Peak Intensity
- Proteomic Alteration
- Surface Enhance Laser Desorption Ionization
- Sample Collection Procedure