Articles

Protein expression analysis ofChlamydia pneumoniae persistence by combined surface-enhanced laser desorption ionization time-of-flight mass spectrometry and two-dimensional polyacrylamide gel electrophoresis

The aim of this study was to examine the protein expression profiles of persistentChlamydia pneumoniae by two-dimensional polyacrylamide gel electrophoresis (2D PAGE) and surface-enhanced laser desorption ionizat...

Authors: Sanghamitra Mukhopadhyay, Saeed A. Jortani, Roland Valdes, Christine Q. Simmons, Erin D. Sullivan, Richard D. Miller and James T. Summersgill

Genistein-induced proteome changes in the human endometrial carcinoma cell line, ishikawa

Epidemiological studies have shown that Asian populations display a lower incidence of hormone-dependant cancers, cardiovascular disease, osteoporosis, and menopausal ailments compared to Western societies. Av...

Authors: Nicki Konstantakopoulos, Martin R. Larsen, Ian G. Campbell, Michael A. Quinn, Mark S. Baker, Harry M. Georgiou and Gregory E. Rice

Salivary protein profiling in type I diabetes using two-dimensional electrophoresis and mass spectrometry

Owing to its noninvasive collection, saliva is considered as a potent diagnostic fluid. The goal of this study was to investigate the modification of the salivary proteome occurring in type 1 diabetes to highl...

Authors: Christophe Hirtz, François Chevalier, Nicolas Sommerer, Isabelle Raingeard, Jacques Bringer, Michel Rossignol and Dominique Deville de Périere

Mass determination of major plasma proteins by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) serves as a rapid and accurate means to determine masses of proteins independent of their shapes or interactions with...

Authors: Glen L. Hortin and Alan T. Remaley

Assessment of protein stability in cerebrospinal fluid using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry protein profiling

Recent studies have evaluated proper acquisition and storage procedures for the use of serum or plasma for mass spectrometry (MS)-based proteomics. The present study examines the proteome stability of human ce...

Authors: Srikanth Ranganathan, Anna Polshyna, Georgina Nicholl, James Lyons-Weiler and Robert Bowser

Comparison of methods to examine the endogenous peptides of fetal calf serum

There is a great desire to relate the patterns of endogenous peptides in blood to human disease and drug response. The best practices for the preparation of blood fluids for analysis are not clear and also rel...

Authors: Declan Williams, Peihong Zhu, Peter Bowden, Catherine Stacey, Mike McDonell, Paul Kowalski, Jane Marie Kowalski, Ken Evans, Eleftherios P. Diamandis, K. W. Michael Siu and John Marshall

Proteomic analysis of urinary fibrinogen degradation products in patients with urothelial carcinomas

Despite many years of research efforts and continued progress in the identification of urine markers for detection of bladder cancer, none of the markers described to date has been able to replace cystoscopy a...

Authors: Pavel Gromov, Josè M. A. Moreira, Irina Gromova and Julio E. Celis

Lysate array analyses of signal transduction inhibitors in tumor cell lines

We probed the ability of reverse lysate array technology to help explain potential differences in the responses of cancer cells to various small-molecule kinase inhibitors. To understand the antitumor potentia...

Authors: Victor A. Levin, Kenji Tada and Cristian Mircean

Data mining in proteomic mass spectrometry

Data mining application to proteomic data from mass spectrometry has gained much interest in recent years. Advances made in proteomics and mass spectrometry have resulted in considerable amount of data that ca...

Authors: Asha Thomas, Georgia D. Tourassi, Adel S. Elmaghraby, Roland Valdes Jr. and Saeed A. Jortani

Proteomic and genomic technologies for biomarker discovery

Authors: Vathany Kulasingam and Eleftherios P. Diamandis

Clinical proteomics

Authors: Daniel W. Chan

Serum proteomic pattern for predicting recurrence of undifferentiated nasopharyngeal carcinoma after radiotherapy

Although most patients with early-stage nasopharyngeal carcinoma (NPC) can be cured by radiotherapy, there is a high recurrence rate in patients with advanced NPC. We attempted to identify proteomic patterns i...

Authors: Terence C. W. Poon, Shuk Man Chow, Brigette B. Y. Ma, Edwin P. Hui and Anthony T. C. Chan

In-depth analysis of the human CSF proteome using protein prefractionation

The identification of disease markers in human body fluids requires an extensive and thorough analysis of its protein constituents. In the present study, we have extended our analysis of the human cerebrospina...

Authors: Giuseppina Maccarrone, Isabel Birg, Eva Malisch, Marcus C. Rosenhagen, Claudia Ditzen, John A. Chakel, Friedrich Mandel, Andreas Reimann, Can-Carlo Doertbudak, Katrin Haegler, Florian Holsboer and Christoph W. Turck

Comparison of protein expression patterns between hepatocellular carcinoma cell lines and a hepatoblastoma cell line

Hepatocellular carcinoma (HCC) and hepatoblastoma (HB) are malignancies of the liver with different etiologies, but the HB cell line HepG2 has been frequently used in various studies of HCC. In this study, we ...

Authors: Ronald T. K. Pang, Terence C. W. Poon, Nathalie Wong, Paul B. S. Lai, Navy L. Y. Wong, Charles M. L. Chan, Jasmine W. S. Yu, Anthony T. C. Chan and Joseph J. Y. Sung

Evaluation of clinical colon carcinoma using activity-based proteomic profiling

The evaluation of clinical tumor tissues is a valuable approach to discovering novel drug targets because of the direct relevance of human samples. We used activity-based proteomic profiling (ABPP) to study th...

Authors: Melissa C. Zhang, Jiangyue Wu, Kristin G. Ardlie and Emme C. K. Lin

Antibody-based tissue profiling as a tool for clinical proteomics

Here, we show a strategy for high-throughput antibody-based tissue profiling with the aim to create an atlas of protein expression patterns in normal human tissues and cancer tissues representing the 20 most p...

Authors: Caroline Kampf, Ann-Catrin Andersson, Kenneth Wester, Erik Björling, Mathias Uhlen and Fredrik Ponten

Antiproliferative heparan sulfate inhibiting hyaluronan and transforming growth factor-β expression in human lung fibroblast cells

The objective of this study was to examine the effects of heparan sulfate (HS) on factors involved in the remodeling of connective tissue observed in patients with fibrotic respiratory disorders such as asthma...

Authors: Kristoffer Larsen, Johan Malmström, Ellen Tufvesson, György Marko-Varga and Gunilla Westergren-Thorsson

Technical evaluation of MALDI-TOF mass spectrometry for quantitative proteomic profiling matrix formulation and application

Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) has been recently used to identify disease markers by directly profiling and quantifying the peptide/proteins in bi...

Authors: Ronald T. K. Pang, Philip J. Johnson, Charles M. L. Chan, Ebenezer K. C. Kong, Anthony T. C. Chan, Joseph J. Y. Sung and Terence C. W. Poon

The discovery of stannin in rat dorsal root ganglia using an integrated proteohistological approach

The use of proteomic analysis to discover proteins (previously identified or unknown) in a tissue sample is a valuable tool. However, there is a limit to the extent one can validate a discovery with any single...

Authors: Stanley M. Belkowski, Raymond W. Colburn, Dennis J. Stone, Christopher R. Van Besien, Deborah Polkovitch, Patricia Andrade-Gordon and Michael R. D'Andrea

New targets for an old drug

Methotrexate has been a clinical agent used in cancer, immunosuppression, rheumatoid arthritis, and other highly proliferative diseases for many years, yet its underlying molecular mechanism of action in these...

Authors: Jacek J. Slon-Usakiewicz, Andrew Pasternak, Neil Reid and Leticia M. Toledo-Sherman

Development of competitive ELISA for neosporosis by employing immunoproteomics

In this study, proteomics was used to explore the antigenic proteins that are involved in cross-reactivity during serodiagnosis between Neospora caninum (N. caninum) and Toxoplasma gondii (T. gondii). Competitive...

Authors: Yong-seung Shin, Eung-goo Lee, Gee-wook Shin, Young-rim Kim, Eun-young Lee, Jae-hoon Kim, Hwan Jang, Dae-yong Kim, Yong-hwan Kim, Gon-sup Kim, Myung-deuk Suh and Tae-sung Jung

Analysis of the human serum proteome

Changes in serum proteins that signal histopathological states, such as cancer, are useful diagnostic and prognostic biomarkers. Unfortunately, the large dynamic concentration range of proteins in serum makes ...

Authors: King C. Chan, David A. Lucas, Denise Hise, Carl F. Schaefer, Zhen Xiao, George M. Janini, Kenneth H. Buetow, Haleem J. Issaq, Timothy D. Veenstra and Thomas P. Conrads

An investigation of plasma collection, stabilization, and storage procedures for proteomic analysis of clinical samples

In order to evaluate the critical components of the process necessary to preserve clinical plasma samples collected at research sites for proteomic analysis, various collection and preservation protocols with ...

Authors: Jeffrey D. Hulmes, Deidra Bethea, Keith Ho, Shu-Pang Huang, Deborah L. Ricci, Gregory J. Opiteck and Stanley A. Hefta

Application of sector protein microarrays to clinical samples

Many protein functions are conferred by posttranslational modifications, which allow proteins to perform specific cellular tasks. Protein microarrays enable specific detection of posttranslational modification...

Authors: Virginia Espina, Emanuel F. Petricoin III, Lance A. Liotta and David Geho

Development of multiplexed protein profiling and detection using near infrared detection of reverse-phase protein microarrays

Protein microarrays have been recently employed for signal pathway profiling and high-throughput protein expression analysis. Reversephase arrays, where the array consists of immobilized analytes and lysates h...

Authors: Valerie S. Calvert, Yihui Tang, Vince Boveia, Julie Wulfkuhle, Amy Schutz-Geschwender, D. Michael Olive, Lance A. Liotta and Emanuel F. Petricoin III

Differential phosphoprofiles of EGF and EGFR kinase inhibitor-treated human tumor cells and mouse xenografts

The purpose of this phospho-proteomics study was to demonstrate the broad analysis of cellular protein phosphorylation in cells and tissue as a means to monitor changes in cellular states. As a cancer model, h...

Authors: David R. Stover, Jennifer Caldwell, Jarrod Marto, Karen Root, Juergan Mestan, Michael Stumm, Olga Ornatsky, Chris Orsi, Nina Radosevic, Linda Liao, Doriano Fabbro and Michael F. Moran

New targets for an old drug

Methotrexate has been a clinical agent used in cancer, immunosuppression, rheumatoid arthritis and other highly proliferative diseases for many years, yet its underlying molecular mechanism of action in these ...

Authors: Leticia M. Toledo-Sherman, Leroi Desouza, Christopher M. Hosfield, Linda Liao, Kelly Boutillier, Paul Taylor, Shane Climie, Linda McBroom-Cerajewski and Michael F. Moran

Unbound free fatty acid concentrations are increased in cardiac ischemia

Monitoring increased plasma unbound free fatty acid (FFA_u) concentrations has been proposed as a biomarker for myocardial ischemia. In the current study, 30 acute coronary syndrome (ACS) patients presenting in th...

Authors: Fred S. Apple, Alan M. Kleinfeld and Jesse Adams III

Skeletal muscle expression of creatine kinase-B in end-stage renal disease

The tissue specificity of creatine kinase (CK-MB) has been shown not to be absolute for the myocardium. Unexplained increases of plasma creatine kinase (CK-MB) occur in patients with skeletal muscle disease, c...

Authors: Vincent Ricchiuti, Ellen M. Voss, Arthur Ney, Mark Odland and Fred S. Apple

Clinical proteomics

Authors: Lance A. Liotta and Emanuel F. Petricoin III

Selected expression profiling of full-length proteins and their variants in human plasma

With increased interest in clinical proteomics—the comparative investigation of differential protein expression patterns for use in the diagnostic and prognostic assessment of disease states—the demand for tec...

Authors: Urban A. Kiernan, Dobrin Nedelkov, Kemmons A. Tubbs, Eric E. Niederkofler and Randall W. Nelson

Moving forward with clinical proteomics

Authors: Sam Hanash