Skip to main content
Fig. 1 | Clinical Proteomics

Fig. 1

From: Liquid Chromatography/Mass Spectrometry (LC/MS)-Based Glycoproteomics Technologies for Cancer Biomarker Discovery

Fig. 1

Methods to capture glycopeptides. (1) Lectin/antibody-mediated affinity chromatography, (2) hydrazide chemistry-based covalent capturing, (3) BEMAD and subsequent disulfide formation, (4, 5) metabolic incorporation of azidosugar and ligation of biotin/FLAG tags via (4) Staudinger ligation or (5) Huisgen cycloaddition, and (6) enzymatic addition of a keto-derivative of Gal onto O-GlcNAc and subsequent incorporation of biotin tag. Black hexagon sugar chain, gray hexagon Gal, white hexagon O-GlcNAc

Back to article page

Clinical Proteomics

ISSN: 1559-0275