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Featured article: Neoplastic cell enrichment of tumor tissues using coring and laser microdissection for proteomic and genomic analyses of pancreatic ductal adenocarcinoma

One of the challenges in the proteogenomic analysis of cancer tissues, especially in pancreatic ductal adenocarcinoma (PDAC), is the low and heterogeneous composition of neoplastic cellularity in bulk tumors. In this study, we enriched neoplastic cells from bulk tumor tissues using coring and laser microdissection (LMD) sampling techniques. We assessed the protein and KRAS mutation changes associated with specimens obtained by these sampling techniques and evaluated the fraction of neoplastic cells in PDAC for proteomic and genomic analyses. We showed that both bulk and coring sampling techniques gave similar proteogenomic signatures, while the greatest enrichment of neoplastic cellularity could be obtained with the LMD technique.


Archival content

In 2011, Clinical Proteomics converted from a subscription publication to a fully open access journal. The journal's back content can be viewed on SpringerLink.

Aims and scope

Clinical Proteomics encompasses all aspects of translational proteomics. Special emphasis will be placed on the application of proteomic technology to all aspects of clinical research and molecular medicine. The journal is committed to rapid scientific review and timely publication of submitted manuscripts.

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Editor's profile

Dr Daniel W Chan  is currently Professor of Pathology, Oncology, Urology, and Radiology at Johns Hopkins Medical Institutions, and Director of the Clinical Chemistry Division, Co-Director at the Pathology Core Laboratory, and Director at the Center for Biomarker Discovery and Translation at the Johns Hopkins University School of Medicine. He has worked extensively on the development and application of proteomic and immunologic techniques in the understanding of cancer. As the author of five books and over 300 articles, Dr Chan has become established as a leading expert in clinical proteomics and cancer research. Dr Chan is an active member of US Human Proteome Organization (USHUPO), American Association for Cancer Research (AACR) and American Association for Clinical Chemistry (AACC).

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