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Fig. 2 | Clinical Proteomics

Fig. 2

From: Liquid Chromatography/Mass Spectrometry (LC/MS)-Based Glycoproteomics Technologies for Cancer Biomarker Discovery

Fig. 2

Identification of glycopeptides after deglycosylation. (1) Deglycosylation of N-glycan by PNGase and concomitant conversion of Asn to Asp that allows a glycosylation site-specific incorporation of 18O. (2) BEMAD. (3) Partial deglycosylation by Endo D/H treatment with exoglycosidases. Proximal GlcNAc remains on the modified Asn with/without core Fuc. Black diamond Sia, black circle Gal, black square GlcNAc, white circle Man, white triangle Fuc

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Clinical Proteomics

ISSN: 1559-0275