Fig. 1
From: Blood Levels of Carbonic Anhydrase 9 Correlate with Clear Cell Renal Cell Carcinoma Activity
Expression of carbonic anhydrase 9 (CA9) in human renal cell carcinoma cell lines and tumors. a Cell lysates of clones derived from human renal cell carcinoma cell lines 786-O, UMRC2, and UMRC6 stably transfected with vector control plasmid (lanes 1, 3, and 5) or plasmids expressing VHL30 (lanes 2a and 4) or VHL19 (lane 6) were immunoblotted for HIF1a, HIF2a, VHL, and CA9 as indicated. Actin was used as loading control. b qRT-PCR of CA9 message from same cell lines. c, A Fold increase of CA9 in clear cell human RCC tumor (T) compared to normal (N) matched kidney tissue. c, B Absolute values of expression of CA9 in RCC tumor (T) compared to normal (N) matched kidney tissue. d Relative tissue expression of CA9 in adult human tissues