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Fig. 1 | Clinical Proteomics

Fig. 1

From: Cancer Site-Specific Isoforms of ENOX2 (tNOX), A Cancer-Specific Cell Surface Oxidase

Fig. 1

2-D gel western blot of ENOX2 isoforms comparing pooled non-cancer (a) and pooled cancer (b) patient sera. ENOX2-enriched from pooled cancer sera (major carcinomas) fractions (b) compared to non-cancer (a). The approximate location of unreactive (at background) albumin is labeled for comparison. ENOX2 isoforms are restricted to quadrants I and IV. Detection used recombinant scFv-S (S-tag peptide: His-Gln-Thr-Ala-Ala-Ala-Lys-Phe-Gln-Arg-Gln-His) antibody visualized with alkaline phosphatase-linked S protein (S-AP). The >10 ENOX2 isoforms (b) of pooled sera are absent from non-cancer (a) and are cancer site-specific as indicated in Fig. 2

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