Fig. 5
From: Detection of Autoantibodies to Annexin A11 in Different Types of Human Cancer
Absorption of autoantibodies with recombinant GST-ANXA11N. Sera were diluted in the blocking buffer, and incubated for 2 h at room temperature with GST-ANXA11N recombinant protein. This mixture was centrifuged at 10,000 ×g for 10 min, and the supernatant was then used for the analysis by ELISA with GST-ANXA11N recombinant protein as coating antigen. N1, one healthy women serum, negative control; others, positive sera (N2, N3, positive healthy women sera). There is a significant difference between the autoantibody titers of pre- and post-incubations (P < 0.001)