Table 1 Summary of the description and applications of most commonly used ProteinChips in SELDI-TOF MS proteomic profiling
Array | Description | Applications and requirements |
|---|---|---|
IMAC30 | Immobilized metal affinity capture (IMAC), with nitriloacetic acid surface | Transition metal activation is needed prior to use. Phosphorylated proteins and polyhistidine-tagged proteins capture. Protein profiling and identification |
CM10 (WCX) | Weak cation exchanger with negatively charged carboxylated surface | Protein profiling and identification |
Q10 (SAX) | Strong anion exchanger with positively charged quaternary amine surface | Protein profiling and identification |
H50 | Hydrophobic interaction surface | Protein profiling and identification |
Reverse phase | ||
NP20 | Normal phase surface | Quality control assay and protein molecular weight validation |
PS10, PS20, RS100 | Pre-activated surfaces | Specific protein–protein binding (e.g., protein–ligand or antibody–antigen binding) |