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Table 1 Cervical mucous proteins identified through proteomic analyses

From: Characterization of the Human Cervical Mucous Proteome

Protein name

Acc. no.a

MW (kDa)

pI

MASCOT score

Matched peptides

Sequence coverage (%)

Methodb

Spot no.c

14-3-3 protein epsilon

P62258

29.1

4.63

92

3

10

T

51

14-3-3 protein sigma

P31947

27.7

4.68

71

3

10

T

51

14-3-3 protein zeta/delta

P63104

27.7

4.73

181

5

25

T

50

6-phosphogluconate dehydrogenase

P52209

53.1

6.80

51

2

5

T,G

46

Alcohol dehydrogenase [NADP+]d

P14550

36.5

6.32

75

2

6

T

122

Alpha-1-acid glycoprotein 1 precursore

P02763

23.4

4.93

194

6

34

T,G

56

Alpha-1-antichymotrypsin precursore

P01011

47.6

5.33

285

7

21

T

98–100

Alpha-1-antitrypsin precursore

P01009

46.7

5.37

1092

16

48

T,G, D

45, 57, 59, 97, 123, d19

Alpha-enolase

P06733

47.1

7.01

338

9

19

T,G

46–47, 80

Annexin A1

P04083

38.7

6.57

1893

12

43

T,G, D

35–36, 85, 124, d78–82, d91

Annexin A2

P07355

38.5

7.57

573

13

43

T,G, D

82, 111–112, d37, d45–47, d73, d85

Annexin A3

P12429

36.4

5.63

758

12

42

T,G, D

32–33, 84, d88, d96

Annexin A5

P08758

35.9

4.94

167

7

25

T,G

31

Antileukoproteinase 1 precursor

P03973

14.3

9.11

54

2

6

G

 

Anterior gradient protein 2 homolog precursor

O95994

19.9

9.03

126

4

26

T,G

118–119

Apolipoprotein A-I precursore

P02647

30.8

5.56

1464

16

56

T,G

30, 49, 106–109

Arginase-1

P05089

34.7

6.72

36

3

10

T

125

ATP synthase subunit beta

P06576

56.5

5.26

64

2

4

D

d51

Beta-actine

P60709

41.7

5.29

926

10

34

T,G

58, 96

Calregulind

P27797

48.1

4.29

92

3

8

T, D

101, d48

Carbonic anhydrase 1

P00915

28.9

6.59

133

5

22

T, D

34, 53, 54, d72

Catalase

P04040

59.7

6.9

38

1

1

D

d13

Cathelicidin antimicrobial peptide precursord

P49913

19.3

9.48

135

2

10

T, G

119–120

Ceruloplasmin precursor

P00450

122.1

5.44

124

4

4

T,G, D

69, d53

Complement C3 precursor

1637

187.0

6.02

254

15

11

T,G, D

34, 43, d5, d7

Complement C4-A precursor

1618

192.7

6.65

60

2

1

D

d38

Complement factor B precursor

P00751

85.5

6.67

57

4

5

G

 

Cystatin-A

P01040

11.0

5.38

126

2

30

T,G

21, 24

Cystatin-B

P04080

11.1

6.96

85

2

24

G

 

Elongation factor 1-alpha 1

P68104

50.1

9.10

70

3

6

T

87

Endoplasmin precursor

P14625

92.4

4.76

168

6

8

T, D

62, d50

F-actin-capping protein subunit alpha-1

P52907

32.9

5.45

43

1

3

T

32

Fatty-acid-binding protein, epidermal

Q01469

15.2

6.60

315

6

40

T,G

10

Flavin reductased

P30043

22.1

7.13

35

1

7

T

121

Fructose-bisphosphate aldolase A

P04075

39.4

8.30

44

1

3

D

d44

Glutamine synthetased

P15104

42.0

6.43

99

5

19

G

 

Glutathione-S-transferase Pe

P09211

23.3

5.43

73

2

13

T,G

29

Glyceraldehyde-3-phosphate dehydrogenase

P04406

36.0

8.57

168

6

25

T,G

37, 48

Haptoglobin precursor

P00738

45.2

6.13

226

5

14

T,G

72

Heat-shock 70 kDa protein 1

P08107

69.9

5.48

311

11

17

T, D

126, d19

Heat-shock 70 kDa protein 1 Ld

P34931

70.3

5.76

153

7

10

T

126

Heat-shock 70 kDa protein 5

P11021

72.3

5.07

261

10

20

T

64

Heat-shock 70 kDa protein 6d

P17066

70.9

5.81

125

6

7

T

126

Heat-shock 70 kDa protein 8

P11142

70.9

5.37

240

7

16

T, D

79, 126, d22

Heat-shock protein beta-1

P04792

22.8

5.98

51

1

4

G

 

Heat-shock protein HSP 90-alpha

P07900

84.6

4.94

354

10

16

T

63

Heat-shock protein HSP 90-beta

P08238

83.2

4.97

177

6

9

T

63

Hemoglobin subunit alpha

P69905

15.2

8.72

1787

7

68

G, D

1–3, 13, 22, 78, d81

Hemoglobin subunit beta

P68871

16.0

6.75

4704

12

95

T,G, D

1, 3–6, 8, 13, 34, 70, d61

Hemoglobin subunit delta

P02042

16.0

7.85

2495

7

46

G

 

Hemopexin precursor

P02790

51.6

6.55

43

2

4

T

 

Histone H4

P62805

11.4

11.40

40

2

17

G

 

Ig alpha-1 chain C region

P01876

37.6

6.08

251

6

25

T,G

70

Ig gamma-1 chain C region

P01857

36.0

8.46

1881

10

37

T,G

38, 66, 67, 68

Ig gamma-2 chain C region

P01859

35.9

7.66

745

6

21

T,G

 

Ig heavy chain V-III region BRO

P01766

13.2

6.45

49

1

15

G

 

Ig kappa chain V-I region Lay

P01605

11.8

7.96

46

1

8

G

 

Ig kappa chain C regione

P01834

11.6

5.58

3002

5

80

T,G

28, 30, 52–55, 76, 113

Ig kappa chain V-I region OU

P01606

11.8

9.94

67

3

24

G

 

Ig kappa chain V-II region TEW

P01617

12.3

5.69

89

3

34

G

 

Ig kappa chain V-III region B6

P01619

11.6

9.34

64

1

16

G

 

Ig kappa chain V-III region SIE

P01620

11.8

8.70

407

3

39

T,G

55, 76

Ig kappa chain V-III region VG precursor (Fragment)

P04433

12.6

4.85

42

2

15

G

 

Ig kappa chain V-IV region precursor (Fragment)

P06312

13.4

5.09

45

1

7

G

 

Ig lambda chain C regions

P01842

11.2

6.92

597

5

75

G

 

Ig lambda chain V-I region HA

P01700

11.9

9.07

39

2

18

G

 

IgGFc-binding protein precursor

4878

571.6

5.12

455

18

4

T,G

102, 127

ITI heavy chain H4

Q14624

103.3

6.51

29

2

2

D

d53

Isocitrate dehydrogenase [NAD] subunit alphad

P50213

39.6

6.47

51

1

2

T

 

Lactoferrin

P02788

78.1

8.50

110

5

8

T,G, D

74, d3

Lysozyme C precursor

P61626

16.5

9.38

335

4

24

T,G

28, 70, 77

Myeloblastin precursor

P24158

27.8

8.72

136

6

11

T,G, D

86, d21

Neutrophil defensin 1 precursor

P59665

10.2

5.71

471

3

26

G

 

Neutrophil gelatinase-associated lipocalin precursor

P80188

22.5

9.02

494

5

32

T,G

75, 91, 92

Peptidyl-prolyl cis–trans isomerase A

P62937

18.0

7.68

132

4

28

T,G, D

116, 117, d70

Peroxiredoxin-1

Q06830

22.1

8.27

52

1

5

D

d62

Peroxiredoxin-5

P30044

22.0

8.85

57

3

18

T

128

PH domain leucine-rich repeat protein phosphatased

O60346

185

5.88

43

2

43

G

 

Phosphoglycerate kinase 1

P00558

44.6

8.30

190

8

26

T,G

38, 110

Plastin-1d

Q14651

70.3

5.33

41

2

2

G

 

Plastin-2e

P13796

70.2

5.20

345

14

13

T,G

65

Poly-Ig receptore

P01833

83.2

5.58

451

7

11

T,G

62, 63

Profilin-1

P07737

15

8.44

163

4

37

G, D

d1–2

Proteasome subunit alpha type 5d

P28066

26.3

4.74

66

2

10

T

129

Protein disulfide isomerase precursor

P07237

57.1

4.76

197

6

12

T, D

60, d14, d49

Protein S100-A11

P31949

11.7

6.56

48

2

8

T

13

Protein S100-A6d

P06703

10.1

5.33

50

3

46

T,G

23

Protein S100-A7

P31151

11.5

6.27

194

7

61

T,G

11

Protein S100-A8e

P05109

10.8

6.51

1083

8

50

T,G, D

6, 7, 9, 12, 27, 130, d66

Protein S100-A9e

P06702

13.2

5.71

1526

7

63

T,G

8, 15–16, 19–20, 25, 45

Protein S100-A12

P80511

10.6

41

2

  

D

d64

Pyruvate kinase isozymes M1/M2

P14618

57.9

7.96

158

5

10

T

73

Serpin B1

P30740

42.7

5.90

707

14

43

T,G, D

42, 93, 131, d16, d26

Serpin B13

Q9UIV8

44.2

5.48

107

6

22

T, D

72, d25

Serpin B3e

P29508

44.5

6.35

2296

19

46

T,G, D

39–42, 71, 88–89, 91–92, 132–134, d17, d26–29

Serum albumin precursor

P02768

69.3

5.92

15383

119

71

T,G, D

58, 71, 83, 88, d15

Small proline-rich protein 3

Q9UBC9

18.1

8.86

94

5

23

G

 

Superoxide dismutase [Mn]

P04179

24.7

8.35

78

3

13

T

118

Thioredoxin

P10599

11.7

4.82

49

1

12

G, D

d60

Transaldolase

P37837

37.5

6.36

154

2

6

T

90, 94–95

Transferrine

P02787

77.0

6.81

855

20

32

T,G

61

Transthyretin precursor

P02766

15.9

5.52

228

6

63

T

18, 26

Triosephosphate isomerase

P60174

26.7

6.45

83

4

19

T

52

Tropomyosin-1 alpha chaine

P09493

32.6

4.69

129

3

9

T

105

Tropomyosin beta chaine

P07951

32.8

4.66

153

3

9

T

104

Ubiquitin

P62988

8.6

6.56

84

2

32

T

14

Vitronectin precursor

P04004

54.2

5.22

59

1

2

T

100

  1. aSwiss-Prot protein accession number
  2. bT 2D gel data, G GeLC-MS/MS data, D 2D-gel after depletion
  3. cXX and dXX represent the numbers shown in Figs. 1a and 2b, respectively
  4. dProteins not found in previously reported CVF and cervical mucous studies (Refs. [3–9])
  5. ePTM proteins identified in this study. The region denoting heat-shock proteins and Immunoglobulins also showed glycosylation as seen in Fig. 4a
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Clinical Proteomics

ISSN: 1559-0275