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Fig. 2 | Clinical Proteomics

Fig. 2

From: Novel In Situ Collection of Tumor Interstitial Fluid from a Head and Neck Squamous Carcinoma Reveals a Unique Proteome with Diagnostic Potential

Fig. 2

Proteomic analysis strategy for identifying proteins from TIF. High abundance proteins in TIF were removed using a MARS column. Remaining proteins were digested with trypsin to generate peptides. The peptides were subjected to three dimensions of fractionation using isoelectric focusing, strong cation exchange chromatography, and reversed-phase chromatography prior to tandem mass spectrometry analysis. MS/MS data was searched against a human database using MaxQuant

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Clinical Proteomics

ISSN: 1559-0275