Table 1 Overlay of HNSCC TIF identified proteins with previously determined protein biomarkers for HNSCC assayed from bio-fluids
Source of protein biomarker discovery | Proteomics approach (separation and quantification) | Verified biomarkers (accession no.) | Biomarker verification methods/statistical analyses | Biomarker potential characteristics | Ref. | No. of unique peptides identified in HNSCC TIF | Molecular weight (kDa) | Sequence coverage (%) |
|---|---|---|---|---|---|---|---|---|
Orthotopic xenograft mouse model OSCC/serum analysis | DIGE and iTRAQ/2DLC | EGFR (A8K2T7) | ELISA | Serum biomarkers for OSCC tissue and tumor invasion | [21] | 0 | 134 | 0 |
Western blot | ||||||||
IHC | ||||||||
OSCC saliva | SCX/RP HPLC and 2D GE | Mac-2 binding protein (Q08380) | Saliva biomarkers for OSCC | [22] | 6 | 65.3 | 10.4 | |
S100-A9 (P06702) | ELISA | 5 | 13.2 | 56.1 | ||||
CD59 (P13987) | Western blot | 1 | 14.2 | 9.4 | ||||
Profilin (P07737) | Receiver operator characteristics analysis | 7 | 15.1 | 59.3 | ||||
Catalase (P04040) | 31 | 59.8 | 60 | |||||
HNSCC saliva | 2D DIGE | S100-A9 (P06702) | Western blot | Early detection of HNSCC | [23] | 5 | 13.2 | 56.1 |
Western blot | ||||||||
IHC | ||||||||
OSCC cell line secretome | 1D SDS-PAGE | Mac-2 binding protein (Q08380) | ELISA on serum | Mac-2 BP as serum biomarker | [24] | 6 | 65.3 | 10.4 |
Receiver operator characteristics analysis | ||||||||
Mac-2 BP siRNA and assay cell proliferation, invasion, and migration |