Fig. 1
From: Proteomics of AML1/ETO Target Proteins: AML1–ETO Targets a C/EBP–NM23 Pathway
Western blot analysis for inducible AML1–ETO expression. a U937Z/A-E cells were induced with 100 μM ZnSO4 for 6 h, lysed in RIPA buffer, and analyzed for AML1–ETO expression by Western blot. U937 cells were also induced and used as control. In vitro translated AML1–ETO (lane 1) was used as a positive control. As seen in lane 5, AML1–ETO expression is increased after Zn induction. b U937T/A-E cells were induced by removing tetracycline from the medium for 24, 48, 72, and 94 h. Cells were lysed in RIPA buffer and blotted for AML1–ETO. U937T cells containing the empty vector were used as a control. AML1–ETO expression level was increased after 24 h of tet-off induction (lane 4) and was continuously detectable even after 90 h of induction (lanes 5–7)