Fig. 2
From: Proteomics of AML1/ETO Target Proteins: AML1–ETO Targets a C/EBP–NM23 Pathway
Two-dimensional gel electrophoresis to identify AML1–ETO target proteins. U937Z/A-E cells were induced with Zn for 6 or 12 h, and samples were lysed in urea lysis buffer, and lysates were separated in one- and two-dimensional gel electrophoresis and were stained with colloidal Coomassie blue stain (Sigma, Cat. B-0770) to visualize the protein spots. As a control, U937 cells were also induced with Zn, lysed in urea lysis buffer, separated on 2D gels, and compared with U937Z/A-E gels (data not shown)