Fig. 3
From: Proteomics of AML1/ETO Target Proteins: AML1–ETO Targets a C/EBP–NM23 Pathway
AML1–ETO upregulates the protein expression of NM23. a U937T/A-E cells were induced by removing tetracycline from the medium for 24, 48, and 72 h. Cells were lysed in RIPA lysis buffer; 80 μg protein was separated on 12% SDS-PAGE and blotted for NM23 with anti-NM23-H2 goat polyclonal antibody (Santa Cruz, Cat. sc14790). NM23 expression was upregulated in AML1–ETO-induced cells (lanes 3–5) in comparison with empty vector U937T cells (lane 1) and U937T/A-E cells at 0 h (lane 2) of upper panel. The same blot was stripped and probed for β-tubulin for loading control (lower panel). b Western blot analysis for prohibitin from U937Z/A-E cells using anti-prohibitin antibody shows prohibitin is upregulated in with AML1–ETO expression after 6 h (upper panel). The same blot was stripped and probed for β-tubulin for loading control (lower panel)