Proteomics of AML1/ETO Target Proteins: AML1–ETO Targets a C/EBP–NM23 Pathway

Table 1 AML1–ETO-regulated proteins identified 6 h after Zn induction

Description	Acc. no.	Mass (Da)	Up/down
Homo sapiens cDNA clone IMAGE 4104570	gi11156428	30,383	Down
Homo sapiens cDNA clone IMAGE 3905254	gi10407098	34,977	Down
cDNA FLJ11534 FIS, clone HEMBA1002679	Q9HAJ5	71,672	Down
Phosphoglycerate kinase	KIHUG	44,985	Down
Prohibitin	I52690	29,843	Up
Uncharacterized hematopoietic stem/progenitor cells protein MDS032	Q9NZ43	29,440	Up
mRNA for DNA helicase Q1	BAA07200	74,392	Down
TRIP230	015154	228,116	Up
Nucleoside-diphosphate kinase-NM23-H1g	A33386	17,309	Up
dUTP pyrophosphatase	G02777	17,908	Down
HSP27	E980237	22,427	Up
HSTROPCR (Tropomyosin)	CAA28258	28,185	Down

Identification of AML1–ETO target proteins by MALDI-TOF mass spectrometry. After comparing gels for uninduced U937Z/A-E (gel not shown) and Zn-induced U937Z/A-E cells using the ProteomWeaver Software (Definiens), differentially expressed protein spots were excised, digested with trypsin, and identified using mass spectrometry and MASCOT database search. A total of 28 target proteins of AML1–ETO (12 from 6 h and 16 from 12 h Zn-induced condition) are identified. Two-dimensional gel electrophoresis and mass spectrometry were performed from three independent experiments, and only proteins identified in each of those three experiments were considered to be target proteins of AML1–ETO

ISSN: 1559-0275