Proteomics of AML1/ETO Target Proteins: AML1–ETO Targets a C/EBP–NM23 Pathway

Table 2 AML1–ETO-regulated proteins identified 12 h after Zn induction

Description	Acc. no.	Mass (Da)	Up/down
Calreticulin precursor	A37047	48,283	Up
Alpha enolase	ENOA_HUMAN	47,350	Up
Alpha enolase	ENOA_HUMAN	47,350	Up
Alpha enolase	ENOA_HUMAN	47,350	Up
Glyceraldehyde-3-phosphate dehydrogenase	CAA25833	36,202	Up
Glyceraldehyde-3-phosphate dehydrogenase	CAA25833	36,202	Up
Annexin I	LUHU	38,918	Up
Hypothetical protein FLJ35908 (fragment)	Q8NA27	65,077	Up
Alpha-complex protein 1	S58529	37,987	Up
Proteasome alpha 1 subunit, isoform 2	AAH02577	29,822	Up
Triosephosphate isomerase	TPIS_HUMAN	26,807	Up
Phosphoglycerate mutase	PMHUYB	28,900	Up
Probable thioredoxin peroxidase	A46711	22,324	Up
Triosephosphate isomerase	TPIS_HUMAN	26,807	Up
Peptydil-prolyl-cis-trans isomerase A	CYPH_HUMAN	18,098	Up
Glutathion S-transferase P1C human	Q15690	23,583	Up

Identification of AML1–ETO target proteins by MALDI-TOF mass spectrometry. After comparing gels for uninduced U937Z/A-E (gel not shown) and Zn-induced U937Z/A-E cells using the ProteomWeaver Software (Definiens), differentially expressed protein spots were excised, digested with trypsin, and identified using mass spectrometry and MASCOT database search. A total of 28 target proteins of AML1–ETO (12 from 6 h and 16 from 12 h Zn-induced condition) are identified. Two-dimensional gel electrophoresis and mass spectrometry were performed from three independent experiments, and only proteins identified in each of those three experiments were considered to be target proteins of AML1–ETO

ISSN: 1559-0275