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Table 2 Average relative changes in serum concentrations after incubation of serum from four healthy individuals for 4 h at RT

From: Specific Investigation of Sample Handling Effects on Protease Activities and Absolute Serum Concentrations of Various Putative Peptidome Cancer Biomarkers

Peptide

Relative difference (%) ±standard deviation

Serum preparation

Clotting time (h); temperature (°C)

Tube with gel

Tube without gel

Fib-α [605–629]

1; RT

+ 48.3 ± 30

+ 7.4 ± 8

Fib-α [605–629]

24; 5

+18.9 ± 8

−19.0 ±12

C4a [1337–1350]

24; 5

−54.5 ± 17

−55.5 ± 18

Bradykinin

1; RT

−64.3 ± 18

−53.9 ± 23

Des-Arg9-bradykinin

24; 5

−82.9 ± 3

−87.1 ± 5

ITIH4-21

24; 5

+ 151.6 ± 20

+ 298.1 ± 124

ITIH4-22

24; 5

+ 95.3 ± 40

+ 75.4 ± 17

ITIH4-25

24; 5

+ 417.5 ± 99

+ 361.7 ± 108

ITIH4-26

24; 5

+ 35.2 ± 13

+ 30.0 ± 11

ITIH4-27

24; 5

+ 202.5 ± 43

+139.0 ± 52

ITIH4-28

24; 5

+ 90.4 ± 12

+ 127.7 ± 42

ITIH4-29

24; 5

+ 109.4 ± 46

+ 71.9 ± 10

ITIH4-30

1; RT

+ 33.1 ± 23

+ 18.8 ± 14

ITIH4-30

24; 5

−10.9 ± 12

−11.7 ± 20