Fig. 4
From: Membrane Protein Profiling of Human Islets of Langerhans Using Several Extraction Methods
Venn diagram comparing the numbers and overlap of proteins identified after different membrane protein extractions of human pancreatic islets. a The number of proteins identified as plasma membrane proteins according to gene ontology annotation. b The number of proteins predicted with at least one transmembrane helix in their protein sequence. Protein identification, at >99%, required at least two unique peptides, at ≥95% confidence level, detected by LC-FTICR MS/MS, searched against the human Swiss-Prot database using MASCOT and X!Tandem. Hydrophoboc phase the hydrophobic phase of Triton X-114 phase separation, Plasma MPE plasma membrane protein extraction, Cell SPB cell surface protein biotinylation, LPI flow cell lipid-based protein immobilization flow cell