Table 1 Amount of peptides and proteins identified after the different extraction methods and their extraction efficiency of subcellular components
From: Membrane Protein Profiling of Human Islets of Langerhans Using Several Extraction Methods
Extraction method | # Unique proteins | # Unique peptides | Membrane | Plasma membrane | Cell surface | Cytosol | Mitochondrion | E.R. | Golgi | Nucleus | Cytoskeleton | TMH pred. |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Total protein extraction | 1,136 | 9,250 | 34.3% | 15.8% | 1.8% | 24.6% | 18.8% | 9.7% | 6.5% | 28.0% | 9.9% | 12.3% |
Hydrophobic phase | 785 | 5,239 | 64.1% | 30.4% | 4.5% | 15.3% | 18.0% | 15.7% | 12.1% | 19.4% | 6.8% | 36.8% |
Insoluble phase | 804 | 5,630 | 44.4% | 18.7% | 1.6% | 24.9% | 19.3% | 12.8% | 7.0% | 30.0% | 10.4% | 17.4% |
Plasma membrane protein extraction | 386 | 2,780 | 47.4% | 26.9% | 4.9% | 27.2% | 19.2% | 16.3% | 10.1% | 22.0% | 12.7% | 18.4% |
Cell surface protein biotinylation | 329 | 3,077 | 47.1% | 21.6% | 4.3% | 11.9% | 39.5% | 15.5% | 8.2% | 26.1% | 11.2% | 18.5% |
LPI flow cell | 143 | 794 | 62.2% | 26.6% | 3.5% | 25.9% | 3.5% | 30.8% | 13.3% | 13.3% | 7.0% | 42.0% |
Total #, all methods combined | 1,700 | 14,824 | 735 | 343 | 43 | 331 | 295 | 198 | 135 | 441 | 153 | 360 |