Table 2 Identified proteins with receptor function from the human islets of Langerhans
From: Membrane Protein Profiling of Human Islets of Langerhans Using Several Extraction Methods
Protein name | UniProt account no. | Mw (kDa) | Total protein extraction | Cell surface protein biotinylation | Hydrophobic phase | Insoluble phase | Plasma membrane protein extraction | LPI flow cell | GO term | TMH prediction | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Protein ID probability (%) | Unique peptides | Protein ID probability (%) | Unique peptides | Protein ID probability (%) | Unique peptides | Protein ID probability (%) | Unique peptides | Protein ID probability (%) | Unique peptides | Protein ID probability (%) | Unique peptides | Membrane | Plasma membrane | Cell surface | ||||
40S ribosomal protein SA | P08865 | 33 | 100 | 8 | 0 | 0 | 100 | 7 | 100 | 6 | 100 | 3 | 0 | 0 | Y | Y | – | 0 |
alpha-2-Macroglobulin receptor-associated protein | P30533 | 41 | 50 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 100 | 3 | 0 | 0 | Y | Y | Y | 1 |
Aminopeptidase N | P15144 | 110 | 0 | 0 | 0 | 0 | 100 | 12 | 0 | 0 | 0 | 0 | 0 | 0 | Y | Y | – | 1 |
ATP-binding cassette transporter sub-family C member 8 | Q09428 | 177 | 0 | 0 | 0 | 0 | 0 | 0 | 100 | 3 | 0 | 0 | 100 | 3 | Y | Y | – | 6 |
B cell receptor-associated protein 31 | P51572 | 28 | 100 | 7 | 100 | 2 | 100 | 5 | 100 | 7 | 100 | 7 | 100 | 8 | Y | Y | – | 3 |
Cation-dependent mannose-6-phosphate receptor | P20645 | 31 | 0 | 0 | 0 | 0 | 100 | 6 | 0 | 0 | 0 | 0 | 86 | 1 | Y | Y | Y | 1 |
Cation-independent mannose-6-phosphate receptor | P11717 | 274 | 100 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | Y | Y | – | 1 |
CD166 antigen | Q13740 | 65 | 100 | 1 | 0 | 0 | 100 | 17 | 0 | 0 | 0 | 0 | 0 | 0 | Y | Y | – | 1 |
CD44 antigen | P16070 | 82 | 100 | 6 | 0 | 0 | 100 | 4 | 0 | 0 | 100 | 3 | 100 | 3 | Y | Y | Y | 1 |
CD63 antigen | P08962 | 26 | 100 | 2 | 0 | 0 | 100 | 3 | 99 | 1 | 100 | 2 | 0 | 0 | Y | Y | – | 4 |
CD81 antigen | P60033 | 26 | 0 | 0 | 0 | 0 | 100 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | Y | Y | – | 4 |
CD82 antigen | P27701 | 30 | 0 | 0 | 0 | 0 | 100 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | Y | Y | – | 4 |
CD9 antigen | P21926 | 25 | 50 | 1 | 0 | 0 | 100 | 4 | 0 | 0 | 50 | 1 | 0 | 0 | Y | Y | – | 4 |
CD99 antigen | P14209 | 19 | 99 | 1 | 50 | 1 | 100 | 2 | 45 | 0 | 0 | 0 | 83 | 1 | Y | Y | – | 2 |
Claudin-3 | O15551 | 23 | 0 | 0 | 0 | 0 | 100 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | Y | Y | – | 4 |
Endoglin | P17813 | 71 | 0 | 0 | 0 | 0 | 100 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | Y | Y | Y | 1 |
ER lumen protein retaining receptor 1 | P24390 | 25 | 50 | 1 | 0 | 0 | 0 | 0 | 100 | 2 | 0 | 0 | 0 | 0 | Y | – | – | 4 |
gamma-Aminobutyric acid receptor-associated protein-like 2 | P60520 | 14 | 100 | 2 | 0 | 0 | 100 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | Y | – | – | 0 |
G-protein coupled receptor 56 | Q9Y653 | 78 | 0 | 0 | 0 | 0 | 100 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | Y | Y | – | 7 |
High affinity cationic amino acid transporter 1 | P30825 | 68 | 0 | 0 | 0 | 0 | 100 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | Y | Y | – | 4 |
HLA class I histocompatibility antigen, A-68 alpha chain | P01891 | 41 | 0 | 0 | 0 | 0 | 100 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | Y | Y | – | 1 |
HLA class I histocompatibility antigen, A-69 alpha chain | P10316 | 41 | 100 | 2 | 0 | 0 | 100 | 10 | 100 | 2 | 100 | 2 | 83 | 1 | Y | Y | – | 1 |
HLA class I histocompatibility antigen, B-51 alpha chain | P18464 | 41 | 0 | 0 | 0 | 0 | 100 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | Y | Y | – | 1 |
HLA class I histocompatibility antigen, B-8 alpha chain | P30460 | 40 | 50 | 1 | 0 | 0 | 100 | 6 | 50 | 1 | 0 | 0 | 100 | 2 | Y | Y | – | 1 |
Integrin alpha-1 | P56199 | 131 | 0 | 0 | 0 | 0 | 100 | 5 | 0 | 0 | 50 | 1 | 0 | 0 | Y | Y | Y | 1 |
Integrin alpha-2 | P17301 | 129 | 0 | 0 | 0 | 0 | 100 | 16 | 0 | 0 | 0 | 0 | 0 | 0 | Y | Y | Y | 1 |
Integrin alpha-5 | P08648 | 115 | 0 | 0 | 0 | 0 | 100 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | Y | Y | Y | 1 |
Integrin alpha-V | P06756 | 116 | 50 | 1 | 50 | 1 | 100 | 23 | 0 | 0 | 0 | 0 | 0 | 0 | Y | Y | Y | 1 |
Integrin beta-1 | P05556 | 88 | 100 | 10 | 100 | 12 | 100 | 21 | 0 | 0 | 100 | 5 | 100 | 3 | Y | Y | Y | 1 |
Integrin beta-3 | P05106 | 87 | 0 | 0 | 0 | 0 | 100 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | Y | Y | Y | 1 |
Leukocyte surface antigen CD47 | Q08722 | 35 | 7 | 0 | 0 | 0 | 100 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | Y | Y | – | 6 |
Lipolysis-stimulated lipoprotein receptor | Q86X29 | 71 | 0 | 0 | 0 | 0 | 100 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | Y | Y | – | 1 |
Lutheran blood group glycoprotein | P50895 | 67 | 0 | 0 | 0 | 0 | 100 | 6 | 0 | 0 | 21 | 0 | 86 | 1 | Y | Y | Y | 1 |
Lysosome membrane protein 2 | Q14108 | 54 | 100 | 4 | 50 | 1 | 100 | 13 | 100 | 2 | 100 | 6 | 100 | 5 | Y | Y | – | 2 |
Membrane-associated progesterone receptor component 1 | O00264 | 22 | 100 | 4 | 100 | 7 | 100 | 8 | 100 | 2 | 50 | 1 | 100 | 2 | Y | – | – | 1 |
Membrane-associated progesterone receptor component 2 | O15173 | 24 | 100 | 6 | 100 | 5 | 100 | 10 | 100 | 5 | 100 | 3 | 100 | 2 | Y | – | – | 1 |
Mitochondrial import receptor subunit TOM34 | Q15785 | 35 | 98 | 1 | 0 | 0 | 0 | 0 | 100 | 5 | 0 | 0 | 0 | 0 | Y | Y | – | 0 |
Mitochondrial import receptor subunit TOM40 homolog | O96008 | 38 | 100 | 2 | 0 | 0 | 100 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | Y | – | – | 0 |
Mitochondrial import receptor subunit TOM70 | O94826 | 67 | 0 | 0 | 50 | 1 | 0 | 0 | 100 | 6 | 100 | 2 | 0 | 0 | Y | – | – | 2 |
Monocyte differentiation antigen CD14 | P08571 | 40 | 0 | 0 | 0 | 0 | 100 | 6 | 0 | 0 | 50 | 1 | 0 | 0 | Y | Y | – | 0 |
Niemann–Pick C1 protein | O15118 | 142 | 0 | 0 | 0 | 0 | 100 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | Y | Y | – | 2 |
Neutral amino acid transporter B(0) | Q15758 | 57 | 0 | 0 | 0 | 0 | 100 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | Y | Y | – | 9 |
Platelet glycoprotein 4 | P16671 | 53 | 0 | 0 | 0 | 0 | 100 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | Y | Y | Y | 2 |
Poliovirus receptor | P15151 | 45 | 0 | 0 | 0 | 0 | 100 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | Y | Y | Y | 1 |
Prohibitin-2 | Q99623 | 33 | 100 | 13 | 50 | 1 | 100 | 14 | 100 | 12 | 100 | 4 | 86 | 1 | Y | – | – | 0 |
Protein CutA | O60888 | 19 | 100 | 2 | 0 | 0 | 50 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | Y | – | – | 1 |
Receptor expression-enhancing protein 5 | Q00765 | 21 | 100 | 6 | 100 | 3 | 100 | 3 | 0 | 0 | 50 | 1 | 100 | 2 | Y | – | – | 2 |
Receptor-type tyrosine-protein phosphatase-like N | Q16849 | 106 | 0 | 0 | 98 | 1 | 100 | 4 | 50 | 1 | 0 | 0 | 100 | 2 | Y | Y | – | 1 |
Renin receptor | O75787 | 39 | 0 | 0 | 0 | 0 | 100 | 3 | 50 | 10 | 0 | 0 | 0 | 0 | Y | Y | Y | 1 |
Ribosome-binding protein 1 | Q9P2E9 | 152 | 100 | 28 | 100 | 3 | 50 | 1 | 100 | 21 | 100 | 10 | 0 | 0 | Y | – | – | 1 |
Signal recognition particle receptor subunit alpha | P08240 | 70 | 9 | 0 | 0 | 0 | 0 | 0 | 100 | 3 | 0 | 0 | 0 | 0 | Y | – | – | 0 |
Signal recognition particle receptor subunit beta | Q9Y5M8 | 30 | 100 | 2 | 0 | 0 | 100 | 2 | 100 | 7 | 0 | 0 | 100 | 2 | Y | – | – | 1 |
Sortilin | Q99523 | 92 | 0 | 0 | 0 | 0 | 100 | 12 | 0 | 0 | 50 | 1 | 0 | 0 | Y | Y | Y | 1 |
Sortilin-related receptor | Q92673 | 248 | 0 | 0 | 100 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 86 | 1 | Y | Y | – | 1 |
Sushi domain-containing protein 2 | Q9UGT4 | 90 | 0 | 0 | 0 | 0 | 100 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | Y | Y | – | 1 |
Tetraspanin-7 | P41732 | 28 | 100 | 3 | 50 | 1 | 100 | 3 | 0 | 0 | 0 | 0 | 89 | 1 | Y | Y | – | 4 |
Tetraspanin-3 | O60637 | 28 | 0 | 0 | 0 | 0 | 100 | 2 | 0 | 0 | 0 | 0 | 89 | 1 | Y | – | – | 4 |
Transferrin receptor protein 1 | P02786 | 85 | 15 | 0 | 0 | 0 | 100 | 23 | 50 | 1 | 100 | 2 | 0 | 0 | Y | Y | – | 1 |
Translocation protein SEC62 | Q99442 | 46 | 0 | 0 | 0 | 0 | 100 | 3 | 99 | 1 | 0 | 0 | 99 | 1 | Y | – | – | 2 |
Tyrosine-protein kinase-like 7 | Q13308 | 118 | 0 | 0 | 0 | 0 | 100 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | Y | Y | – | 2 |
Tyrosine-protein phosphatase non-receptor type 1 | P18031 | 50 | 0 | 0 | 0 | 0 | 100 | 5 | 50 | 1 | 0 | 0 | 0 | 0 | Y | Y | – | 1 |