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Table 1 Differentially expressed HCC, fibrotic liver nuclear membrane proteins and HepG2 cell line

From: Protein expression profiling of nuclear membrane protein reveals potential biomarker of human hepatocellular carcinoma

Acc

Name

Abb

Thr. pI; mass

Score

Peptide matched

Cellular compartment

PTM

%age cov

Mean normalized volume fibrosis HCC /HepG2

Ratio

Anova (P)

Fold change

      

CF(1), Membrane

       

P30049

ATP synthase subunit delta

ATPD

5.38; 17

58

5

mitochondrion

Isopeptide bond

17%

0.02536

0.00704

0.277

0.02

3.6

Mitochondrion

Ubl conjugation

      

inner membrane

       

P02675

Fibrinogen beta chain

FIBB

8.54; 55

1009

27

Secreted

Disulfide bond

41%

0.03364

0.01628

0.483

0.05

2.1

Glycoprotein

      

Pyrrolidone

      

carboxylic acid

      

P06576

ATP synthase subunit beta

ATPB

5.26; 56

670

18

CF(1), Membrane

Acetylation

29%

0.03448

0.00913

0.264

0.04

3.7

mitochondrion

Phosphoprotein

      

Mitochondrion inner membrane

       

Q549N7

Hemoglobin subunit beta

HBB

6.75;15

484

22

Heptoglobin-

Acetylation

61%

0.22697

0.11803

0.520

0.01

1.9

hemoglobin

Glycation

      

complex

Glycoprotein

 

Phosphoprotein

 

S-nitrosylation

P00167

Cytochrome b5 OS

CYB5A

4.88; 15

176

11

Cytoplasmic

Acetylation

42%

0.01948

0.05883

3.020

0.04

3.0

Endoplasmic

       

reticulum

Membrane

Microsome

P31930

Cytochrome b-c1 complex subunit 1

QCR1

5.94; 52

149

10

Mitochondrion

Acetylation

15%

0.01607

0.00505

0.314

0.05

3.1

inner membrane

Phosphoprotein

      
  1. Accession no. is obtained from SWISS/Prot and percent coverage refers to the percentage of protein sequence coverage, determined by number of matched peptides. Significance was calculated from the Progenesis SameSpots v4.5 (Nonlinear Dynamic, UK), generated statistical analysis. A value of p < 0.05 was considered statistically significant. Functional classes were determined by searching the UniProt database (http://www.uniprot.org).
  2. The proteins were separated on 2DE gels and identified by ESI-QTOF MS/MS.