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Table 2 Further in-depth optimization of monophasic deproteinization using different combinations of methanol/ethanol mix and plasma:solvent ratios of 1:3 and 1:9, respectively

From: Technology platform development for targeted plasma metabolites in human heart failure

   1:3(Plasma:Solvent) 1:9(Plasma:Solvent)
Metabolite name Accesion # 100% Methanol 80% Methanol/Ethanol 50% Methanol/Ethanol 20% Methanol/Ethanol 100% Ethanol 100% Methanol 80% Methanol/Ethanol 50% Methanol/Ethanol 20% Methanol/Ethanol 100% Ethanol
Hexanoic acid HMDB00535 x x x x   x   x   x
Succinate HMDB00254    x x x x   x x x
1-Methylhistamine HMDB00898     x       
Trans −4-Hydrxy-L-proline HMDB00725 x   x x x      
Creatine HMDB00064 x   x x       
L-Malate HMDB00156 x    x x      x
Hypoxathine HMDB00157 x   x x x x   x   x
a-Ketoglutaric acid HMDB00208           
L-lysine HMDB00182           
Uric acid HMDB00289           
L-Arginine HMDB00517           
L-Tyrosine HMDB00158           
Indole-3-propionate HMDB02302           
Citric acid HMDB00094           
Dodecanoic acid HMDB00638 x x x x   x x x   x
Palmitatic acid HMDB00220 x x x x   x x x   x
Linoleatic acid HMDB00673 x   x x   x   x   x
Trans-Vaccenic acid HMDB03231 x x x    x     x
Stearic acid HMDB00827 x x x x   x x x   x
# Targeted Meabolites 11 5 11 13 9 8 3 7 1 9