Figure 3

Estrogenic effects in ovariectomized mouse models. (A) Representative Western blots of ER expression post I-R. (B) Average infarct sizes (INF/AAR) recorded from ovariectomized mice implanted with E2 or placebo pellets. n = 4-6. *: p < 0.05 placebo vs E2; ^#: p < 0.05 KO vs WT. AAR/LV ratios are shown in the supplement Additional file 1: Figure S1. (C) Quantitative RT-PCR of SUR2 transcripts in ovariectomized WT mice implanted with E2 or placebo pellets. The signals were normalized to GAPDH. (D) Representative Western blots in ovariectomized mice implanted with E2 or placebo pellets post I-R. Anti-SUR2 (BNJ-2) was used as the primary antibody (1:1000). Secondary antibody was added at 1:10,000. The blot was stripped and hybridized with GAPDH (1:2000), and the density values were normalized to GAPDH.