Figure 2

TRIS concentration-dependence of the protein extraction efficacy as monitored by Grp78, Ass, and Ldha holds to other proteins. FFPE liver was deparaffinized, and extracted by 100, 200, 300, 400, 500 and 600 mM TRIS–HCl, pH 8.0, respectively, all containing 2% SDS at 90°C for 60 min. (A) Western blotting against Grp78, Ass, and Ldha in FFPE mouse liver extracted by PEBs containing 100 ~ 600 mM TRIS. (B) Densitometric comparison of the band intensities in (A). (C) SDS-PAGE profiling of proteins in FFPE mouse liver tissue extracted by PEBs containing 100-600 mM TRIS. (D) Densitometric comparison of the total band intensities in (C). The mean and SD values of band intensities for three independent experimental triplicates (n = 3) are shown.