Table 1 A comparison of SPE and mSPE methods for urine peptide extraction
From: Optimization for peptide sample preparation for urine peptidomics
SPE method | mSPE method | |
|---|---|---|
Sample processing time | 6 h | 8 h |
Sample processing cost | $12 | $15 |
Peptide quantity as measured by BCA assay | 13.6 μg | 1.2 μg |
Sample Appearance and Impact on LC column | Murky and clogs LC column | Clear and no clogging of LC column |
% peptides identified ompared to the total identified peptides | 18% | 82% |