Skip to main content

Table 2 Running conditions for IEF Phase I

From: Immobilized pH gradient-driven paper-based IEF: a new method for fractionating complex peptide mixtures before MS analysis

S1

Step and hold

500 V

0.5 kV-h

S2

Gradient

1000 V

1.0 kV-h

S3

Gradient

8000 V

13.5kV-h

S4

Step and hold

8000 V

60kV-h

S5

Step and hold

500 V

20 h

Back to article page

Clinical Proteomics

ISSN: 1559-0275