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Figure 1 | Clinical Proteomics

Figure 1

From: Spectral counting assessment of protein dynamic range in cerebrospinal fluid following depletion with plasma-designed immunoaffinity columns

Figure 1

Overview of the workflow used for CSF proteome analysis. A pooled CSF sample was divided into 12 equal aliquots. Each aliquot was subjected to immunoaffinity protein depletion as follows: 14 proteins; albumin only; or were not subjected to depletion (controls). 75 μg of each flow-through (or non-depleted sample) was trypsin-digested and further analyzed by LC-MS/MS. MS raw data files were processed with Mascot Distiller and further analyzed with PeptideProphet algorithm. Protein abundance was calculated with APEX spectral counting method. Right-hand column shows analysis including reversed-phase LC peptide fractionation.

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Clinical Proteomics

ISSN: 1559-0275