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Table 2 Reproducibility of MS1 or MS2 spectral counts following various depletion methods.

From: Spectral counting assessment of protein dynamic range in cerebrospinal fluid following depletion with plasma-designed immunoaffinity columns

 

MS1 scans

MS2 scans

IgY14_1

787

3347

IgY14_2

933

3222

IgY14_3

911

3194

IgYHSA_1

783

2906

IgYHSA_2

778

2870

IgYHSA_3

606

2366

Undepleted_1

903

2372

Undepleted_2

1052

2781

Undepleted_3

1058

2888

  1. Depleted or non-depleted CSF samples were analyzed as triplicates. Number of MS1 and MS2 scans over which the middle 50% of the identified peptides elute are shown for each CSF aliquot.
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Clinical Proteomics

ISSN: 1559-0275